High purity recombinant Cellulase (endo-1,4-β-D-glucanase) (Alkali Stable) (Thermobifida halotolerans) for use in research, biochemical enzyme assays andin vitro diagnostic analysis.

EC 3.2.1.4
CAZy Family: GH6
CAS: 9012-54-8

1,4-beta-D-glucan 4-glucanohydrolase

Recombinant. From Thermobifida halotolerans.
In 3.2 M ammonium sulphate.
Supplied at ~ 150 U/mL. 

Specific activity: ~ 27 U/mg protein (on CM-Cellulose 4M) at pH 8.5 and 60^oC;
                           ~ 16 U/mg protein (on CM-Cellulose 4M) at pH 8.5 and 40^oC. 

Stability: > 4 years at 4^oC.

DESCRIPTION

Cellulase (endo-1,4-β-D-glucanase) (Alkali Stable) (Thermobifida halotolerans)

EC 3.2.1.4
CAZy Family: GH6
CAS: 9012-54-8

Synonyms:
1,4-beta-D-glucan 4-glucanohydrolase

Form:
In 3.2 M Ammonium sulphate.

Stability: 
> 4 years at 4^oC.

Specific activity:
~ 27 U/mg protein (on CM-Cellulose 4M) at pH 8.5 and 60^oC;
~ 16 U/mg protein (on CM-Cellulose 4M) at pH 8.5 and 40^oC. 

Unit definition:
One Unit of cellulase (endo-1,4-β-D-glucanase) activity is defined as the amount of enzyme required to release one μmole of glucose reducing-sugar equivalents per minute from CM-Cellulose 4M (10 mg/mL) in sodium phosphate buffer (100 mM) pH 8.5.

Specificity:
endo-hydrolysis of (1,4)-β-D-glucosidic linkages in cellulose.

Applications:
For use in research.