High purity recombinant exo-polygalacturonase (Yersinia enterocolitica) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.82
CAZy Family: GH28
CAS: 37288-58-7

exo-poly-alpha-galacturonosidase

Recombinant. From Yersinia enterocolitica.
In 3.2 M Ammonium sulphate.
Supplied at ~ 900 U/mL.

Specific activity: ~ 220 U/mg protein (60^oC, pH 6.0 on Polygalacturonic Acid).

Stability: > 4 years at 4^oC.

DESCRIPTION

exo-Polygalacturonase (Yersinia enterocolitica)

EC 3.2.1.82
CAZy Family: GH28
CAS: 37288-58-7

Synonyms:
exo-poly-alpha-galacturonosidase.

Form:
In 3.2 M ammonium sulphate.

Specific activity: 
~ 220 U/mg protein (60^oC, pH 6.0 on Polygalacturonic Acid).

Stability: 
> 4 years at 4^oC.

Unit definition:
One Unit of exo-polygalacturonase activity is defined as the amount of enzyme required to release one μmole of reducing-sugar equivalents per minute from Polygalacturonic Acid (10 mg/mL) in potassium phosphate buffer (100 mM), pH 6.0.

Specificity:
Hydrolysis of pectic acid from the non-reducing end, releasing digalacturonate.

Applications: 
For use in research.