The Succinic Acid test kit is suitable for the specific assay of succinic acid in wine, cheese, eggs, sauce and other food products.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of Succinic Acid in foodstuffs,
feed, wine and other materials

Principle:
(succinyl-CoA synthetase)
(1) Succinic acid + ATP + CoA → ADP + succinyl-CoA + P_i

(pyruvate kinase)
(2) ADP + PEP → ATP + pyruvate

(L-lactate dehydrogenase)
(3) Pyruvate + NADH + H⁺ → NAD⁺ + L-lactic acid

Kit size:  20 assays (manual) / 200 (microplate) 
 / 270 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time:  ~ 6 min
Detection limit:  0.26 mg/L
Application examples: 
Wine, fruit and vegetables, soy sauce, cheese, egg, egg products
and other materials (e.g. biological cultures, samples, etc.)
Method recognition: 
Methods based on this principle have been accepted by EEC

Advantages

• Very competitive price (cost per test)
  
• All reagents stable for > 2 years as supplied
  
• Very rapid reaction (even at room temperature)
  
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  
• Standard included
  
• Extended cofactors stability
  
• Suitable for manual, microplate and auto-analyser formats

EK-SUCC DATA BOOKLET.pdf:
EK-SUCC PRODUCT PERFORMANCE.pdf:
m.catalog.equl.cn.pdf:

Megazyme 琥珀酸检测试剂盒操作视频（K-SUCC）

Megazyme 溶解淀粉 操作视频

Megazyme 试剂盒样品前处理准备操作视频