The Fructan HK test kit is suitable for the specific measurement and analysis of all fructo-oligosaccharides (reducing and non-reducing) and of fructan polysaccharide.

UV-method for the determination of Fructan in foodstuffs,
beverages and other materials

Principle:
(sucrase + maltase)
(1) Sucrose + maltosaccharides + H₂O → D-glucose + D-fructose

(exo-inulinase + endo-inulinase)
(2) Fructan + H₂O → D-glucose + D-fructose

(hexokinase)
(3) D-Glucose + D-fructose + ATP → G-6-P + F-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(4) G-6-P + NADP⁺ → gluconate-6-phosphate + NADPH + H⁺

(phosphoglucose isomerase)
(5) F-6-P ↔ G-6-P

Kit size:  50 assays 
Method:  Spectrophotometric at 340 nm
Total assay time: ~ 90 min
Detection limit:  1-100% of sample weight
Application examples: 
Flours, plant materials (e.g. onion), food products and other materials
Method recognition: 
This method is a modification of AOAC Method 999.03 and AACC
Method 32-32.01

Advantages

• Very cost effective
  
• All reagents stable for > 12 months after preparation
  
• Fructan kits are available only from Megazyme
  
• Simple format
  
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  
• Standard included

EK-FRUCHK Data Booklet.pdf:
EK-FRUCHK Product Performance.pdf:
m.catalog.equl.cn.pdf:

Megazyme果聚糖[HK法]试剂盒FructanHK操作视频（K-FRUCHK）

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