乳果糖快速检测方法—Megazyme乳果糖快速检测试剂盒

Megazyme乳果糖检测试剂盒采用酶法分析原理，可定量检测鲜奶、UHT奶、浓缩乳和奶粉等各种乳品中的乳果糖。

与农业部新标准采用相同的检测原理和方法

该试剂盒采用ISO方法11285：2004，经过改进后，更加快速和灵敏

灵敏度是传统己糖激酶法的两倍

成本低

试剂配制后可稳定保存2年

采用试剂盒形式，包含检测必需的所有酶

提供计算软件，使数据处理更方便

包含标准品

 

The Lactulose Assay Kit is suitable for the specific, rapid and sensitive measurement and analysis of lactulose in milk and milk-based samples. Reagents included in this kit may also be prepared for use in the procedure described by ISO Method 11285:2004.

UV-method for the determination of Lactulose in milk and
foodstuffs containing dairy products

Principle:
(β-galactosidase)
(1) Lactulose + H₂O → D-galactose + D-fructose

(glucose oxidase + catalase + H₂O₂)
(2) D-Glucose + H₂O + O₂ → D-gluconic acid + H₂O₂

(hexokinase)
(3) D-Fructose + ATP → F-6-P + ADP

(phosphoglucose isomerase)
(4) F-6-P → G-6-P

(glucose-6-phosphate dehydrogenase)
(5) G-6-P + NADP⁺ → gluconate-6-phosphate + NADPH + H⁺

(gluconate-6-phosphate dehydrogenase)
(6) Gluconate-6-phosphate + NADP⁺ → ribulose-5-phosphate + NADPH
+ CO₂ + H⁺

Kit size:  50 assays
Method: Spectrophotometric at 340 nm
Reaction time:  ~ 120 min
Detection limit:  4.8 mg/L
Application examples:
Milk, dairy products and foods containing milk
Method recognition:  Novel method

Advantages

• Twice the sensitivity of traditional hexokinase based lactulose methods
  
• Very cost effective
  
• All reagents stable for > 2 years after preparation
  
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  
• Standard included

EK-LACTUL Data Booklet.pdf:
EK-LACTUL FAQ.pdf:
m.catalog.equl.cn.pdf:

Jinpan Megazyme 乳果糖检测试剂盒操作视频 (K-LACTUL)

Megazyme 溶解淀粉 操作视频

Megazyme 试剂盒样品前处理准备操作视频