For the measurement of amylose/amylopectin ratio and content in cereal starches and flours. Based on a Con A precipitation procedure. Content:100 assays per kit

Colourimetric method for the determination of Amylose and
Amylopectin in cereals, food and feed

Principle:
(Con A)
(1) Soluble starch (amylose + amylopectin) →
amylose + amylopectin-Con A
(soluble) (precipitate)

(α-amylase + amyloglucosidase)
(2) Amylose (in solution) + H₂O → D-glucose

(glucose oxidase)
(3) D-Glucose + H₂O + O₂ → D-gluconate + H₂O₂

(peroxidase)
(4) H₂O₂ + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + H₂O

(α-amylase + amyloglucosidase)
(5) Total starch (in solution) + H₂O → D-glucose

Kit size:  100 assays
Method: Spectrophotometric at 510 nm
Total assay time: ~ 120 min
Detection limit:  Amylose 5-95% of total starch content
Application examples: 
Cereal starches, flours, pure starches and foods
Method recognition:  Novel method

Advantages

• Very cost effective (cost per test)
  
• All reagents stable for > 12 months after preparation
  
• Only enzymatic kit available
  
• Accurate and reliable amylose / amylopectin ratio determination
  
• Simple format
  
• Standard included

EK-AMYL Data Booklet.pdf:
EK-AMYL FAQ.pdf:
EK-AMYL Product Performance.pdf:
m.catalog.equl.cn.pdf:

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