Advantages

• Extremely high sensitivity – 2.4-fold increase over Ceralpha (K-CERA)
  
• Very cost effective
  
• All reagents stable for > 2 years after preparation
  
• Very specific
  
• Simple format
  
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  
• Standard included

• Suitable for Maual and auto-analyser formats

Highly sensitive colourimetric method for the determination of α-Amylase in sprout damaged grain

Principle:
(α-amylase)
(1) Ethylidene-G₇-α-PNP + H₂O → Ethylidene-G_X + G_(7-X)-α-PNP

(thermostable α-glucosidase)
(2) G_(7-X)-α-PNP + H₂O → D-glucose + PNP

(alkaline solution)
(3) PNP → phenolate ion (yellow colour)
Note: PNP = 4-nitrophenol

Kit size:  160 / 320 assays (manual) / 640 (auto-analyser)
Method:  Spectrophotometric at 400 nm
Total assay time: ~ 5 min
Detection limit:  0.05 U/mL
Application examples: Sprout damaged wheat grain
Method recognition: Novel method