The D-Sorbitol/Xylitol test kit is suitable for the measurement and analysis of D-sorbitol and/or xylitol in food products.
Suitable for manual, auto-analyser and microplate formats.

Colourimetric method for the determination of D-Sorbitol and
Xylitol in foodstuffs and wine

Principle:
(sorbitol dehydrogenase)
(1) D-Sorbitol + NAD⁺ ↔ D-fructose + NADH + H⁺

(sorbitol dehydrogenase)
(2) Xylitol + NAD⁺ ↔ D-xylulose + NADH + H⁺

(diaphorase)
(3) INT + NADH + H⁺ → NAD⁺ + INT-formazan

Kit size:  58 assays (manual) / 580 (microplate) 
 / 700 (auto-analyser)
Method: Spectrophotometric at 492 nm
Reaction time:  ~ 15 min
Detection limit:  0.20 mg/L
Application examples: 
Diabetic foods (e.g. honey, jam and chocolate), dietetic foods,
chewing gum, candies, fruit juice (e.g. apple juice), ice-cream, sweets,
bakery products (e.g. desserts), marzipan, paper (and cardboard), 
cosmetics, pharmaceuticals and other materials (e.g. biological cultures, 
samples, etc.)
Method recognition: 
Methods based on this principle have been accepted by IFU and AIJN

Advantages

• Each vial of sorbitol dehydrogenase is stable for > 2 months at 4°C after dissolution
  
• No wasted diaphorase solution (stable suspension supplied)
  
• Very competitive price (cost per test)
  
• Reagents stable for > 2 years as supplied
  
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  
• Standard included
  
• Suitable for manual, microplate and auto-analyser formats

EK-SORB DATA BOOKLET.pdf:
EK-SORB FAQ.pdf:
EK-SORB PRODUCT PERFORMANCE.pdf:
m.catalog.equl.cn.pdf:

Sample Pre-treatment Format: