Megazyme’s catalase assay kit provides a simple colourimetric method for the measurement of catalase activity. The method is delivered in a fast and reliable format and may be used to detect catalase activity in various samples, including food, biological and bacterial samples. The calculation method included allows for significant variation in the concentration of the H₂O₂ substrate solution that is employed in the assay which translates into a reduction in the ‘hands on’ time required by the analyst in comparison to other commercial kits.

Colourimetric method for the determination of catalase activity.

Principle:
          (catalase)
(1) 2H₂O₂ → H₂O + O₂ 

                              (peroxidase)
(2) 2H₂O₂ + DHBS + AAP → quinoneimine + 4H₂O
                                                  (purple colour)
Note: 
DHBS = 3,5-Dichloro-2-hydroxybenzenesulfonic acid
AAP = 4-aminoantipyrene

Kit size:                            100 / 200 assays
Method:                            Spectrophotometric at 520 nm
Total assay time:             20 min
Detection limit:                0.5 U/mL
Application examples:    Various food, biological and bacterial samples 
Method recognition:       Novel method