工具包包括大约640个测试样本和控制,包含5个微量滴定板。
 

Infectious Bursal Disease (IBD) affects primarily young chickens. The condition is considered economically significant due its ability to induce profound immunosuppression in chickens often resulting in susceptibility to secondary bacterial and viral infections. The symptoms include depression, anorexia, ruffled feathers, and diarrhea. Mortality may be as high as 50%1. Antibodies to avian viruses in serum can be detected by the Enzyme Linked Immunosorbant Assay (ELISA). The levels of Variant and Classic-type IBD antibodies in serum, as well as the immune status of entire flocks can be monitored using ELISA.
传染性法氏囊病（IBD）的影响主要是年轻的鸡。条件被认为在经济上的重要性，由于它能够诱导常导致易继发细菌和病毒感染鸡的深刻的免疫抑制。症状包括抑郁，厌食，羽毛，和腹泻。死亡率可高达50% 1。血清中的禽流感病毒抗体可通过酶联免疫吸附试验（ELISA）检测。不同的经典血清型IBD抗体水平，以及整个鸡群免疫状态可监测使用ELISA。
Inactivated Variant-type Bursal antigen is bound to a microwell. Diluted sera is added to the microwell and incubated. Any anti-IBD antibodies present in the serum will bind to the IBD on the microwell forming an IBD antigen-antibody complex. After washing away any unbound material, anti-Chicken IgG Alkaline Phosphatase conjugate is added to the microwell. The conjugate will bind to the IBD antigen-antibody complex. After incubation any unbound conjugate is removed by washing. Enzyme substrate is then added to the microwell. The substrate reacts with any Alkaline Phosphatase present and forms a yellow product. In the final step the reaction is stopped, fixing the color. The intensity of the color is measured photometrically at 405 – 410 nm.
不同类型的法氏囊灭活抗原绑定到微孔。稀释的血清加入微孔培养。任何抗IBD抗体的血清中会结合在微孔形成抗原抗体复合物IBD IBD。洗去任何未结合的物质后，抗鸡IgG碱性磷酸酶缀合物加入到微孔。共轭将绑定到IBD抗原抗体复合物。孵育后洗涤除去任何未结合的。酶的底物，然后添加到微孔。基板有碱性磷酸酶存在反应并形成黄色产物。在最后一步反应停止，固定的颜色。颜色的强度是在405 – 410 nm光度测量。