This is a precast gel added with Phos-tag™ Acrylamide in advance. It can be used immediately after opening the package. It contains zinc as a supplemental metal. It has excellent storage stability by its neutral gel buffer. Sharp bands can be obtained.

Features:

1. Phosphorylated- and non-phonphorylated proteins are efficiently separable with sharp bands.
2. Long term stability (stable for 6 months)

Physical Properties:

• Plate size: 100 x 100 x 3 (mm)
• Gel size: 90 x 85 x 1 (mm)
• Well number: 13
• Well volume: 30 μL

Note:

• Please note that prestain marker does not reflect the molecular weight, when a gel containing Phos-tag™ is use. It can only be used as reference of electrophoresis. Please use the result as an index of transcription efficiency at Western blotting. At Western blotting, Zn²⁺ must be sufficiently removed with transfer buffer containing 10 mM EDTA. Please wash out the gel for 10 minutes x 3 times. Subsequently, perform the same procedure for 10 minutes using transfer buffer without EDTA, then transfer the gel. 

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