pEGFP-N2 荧光表达载体-质粒载体-分子生物学


别名 pEGFPN2, pEGFP N2
储存条件 -20℃
单位

保存:-20° C
载体信息:
启动子:  CMV
复制子:  pUCori,f1ori
终止子:  SV40poly(A)
载体别名:pEGFPN2, pEGFP N2
载体分类:哺乳细胞表达载体,荧光蛋白报告载体
载体大小:4737bp
载体标签:C-EGFP
原核抗性:卡那霉素(Kanamycin)
筛选标记:新霉素(Neomycin)
克隆菌株:DH5α
表达宿主:哺乳细胞
培养条件:37℃,LB培养基
5′ 测序引物:CMV-F: 5′-CGCAAATGGGCGGTAGGCGTG-3′
3′ 测序引物:EGFP-N: 5′-CGTCGCCGTCCAGCTCGACCAG-3′
载体简介:
pEGFP-N2 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm;emission maximum = 507 nm.) pEGFP-N2 encodes the GFPmut1 variant which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr

pEGFP-N2 荧光表达载体-质粒载体-分子生物学