Directions
Dissolve 9.4g of the medium in distilled water and adjust the volume to 1,000mL. Sterilize by autoclaving at 121 centigrade for 15 minutes. Cool the sterilized medium to room temperature and add a proper amount of sterile 10% sodium bicarbonate solution. (pH will be 7.1-7.4 at 37 centigrade in an
atmosphere of 5% CO2 in air by adding 12.5-22.0mL of sodium bicarbonate solution) This mixture should be stopper tightly and stored in a cool, dark
place. At the time of use, aseptically add 0.292 g of L-glutamine (sterilized) and the desired amount of serum.
Summary
Eagle’s MEM “Nissui” 1 is a powdered medium prepared according to prescription published by Harry Eagle in 1959. Up to now, such media were not autoclavable because of decomposition or deterioration of the constituents, so that sterilization was performed by filtration using a membrane filter. Nissui Pharmaceutical Co., Ltd. has succeeded in preparing an autoclavable powder medium. This medium is specially manufactured so that constituents of the medium and growth of cells with this medium are not impaired. This medium has a growth-promoting effect for cells such as HeLa or L and other cell lines.
Storage and Expiration
Close tightly and store at 2-5°C in a dark and dry place. May be stored up to one (1) year.
Precautions
Do not use the product after its expiry date. Quality of the product is not warranted after its shelf life.
When if medium or reagent touched eyes or mouth, immediately wash with plenty of water, and consult a physician.
Any medium, reagent and materials must be sterilized by autoclaving or boiling water after use, and then disposed as industrial waste according to the law or regulations related to dispose.

Formula

ComponenComponents	In 9.4 g/L
Sodium Chloride	6,800.0 mg
Potassium Chloride	400.0 mg
Calcium Chloride	200.0 mg
Magnesium Sulfate	93.5 mg
Sodium Dihydrogen Phosphate	115.0 mg
Glucose	1,000.0 mg
L-Arginine Hydrochloride	126.0 mg
L-Cystine Dihydrochloride, H2O	31.4 mg
L-Tyrosine	36.0 mg
L-Histidine Hydrochloride, H2O	42.0 mg
L-Isoleucine	52.0 mg
L-Leucine	52.0 mg
L-Lysine Hydrochloride	73.0 mg
L-Methionine	15.0 mg
L-Phenylalanine	32.0 mg
L-Threonine	48.0 mg
L-Tryptophan	10.0 mg
L-Valine	46.0 mg
Succinic Acid	75.0 mg
Sodium Succinate, 6H2O	100.0 mg
Choline Bitartrate	1.8 mg
Folic Acid	1.0 mg
Inositol	2.0 mg
Nicotinamide	1.0 mg
Calcium Pantothenate	1.0 mg
Pyridoxal Hydrochloride	1.0 mg
Riboflavin	0.1 mg
Thiamin Hydrochloride	1.0 mg
Biotin	0.02 mg
Kanamycin	60.0 mg (Titer)
Phenol Red	6.0 mg