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Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) 
100 assays (manual) / 400 assays (auto-analyser)
Prices exclude VAT
| Content: | (Malt β-glucanase) 100 assays (manual) / 400 (auto-analyser) Or (Lichenase) 100 / 200 assays (manual) / 330 (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | β-Glucanase/Lichenase |
| Assay Format: | Spectrophotometer, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 400 |
| Signal Response: | Increase |
| Limit of Detection: | (Malt β-glucanase) 4.3 x 10-4 U/mL Or (Lichenase) 9.1 x 10-5 U/mL |
| Reproducibility (%): | ~ 3% |
| Total Assay Time: | (Malt β-glucanase) ~ 20 min Or (Lichenase) ~ 10 min |
| Application examples: | Crude malt extracts, industrial enzyme preparations. |
| Method recognition: | Novel method |
The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase.
Mixed linkage β-glucanase (endo-1,3:1,4-β-glucanase) / lichenase (EC 3.2.1.73) acts specifically to release 2-chloro-4-nitrophenol (CNP) from this substrate. The rate of release of CNP is directly related to the β-glucanase/lichenase activity in a sample. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).
Note that the substrate is not hydrolysed by β-glucosidase or cellobiohydrolase. The substrate can be hydrolysed by certain endo-cellulases (e.g. Trichoderma sp.) but this does not result in an increase in absorbance.
Discover more assay kits for enzyme activity measurement.
Data calculators are located in the Documents tab.
- Very cost effective
- All reagents stable for > 2 years
- Specific for endo-1,3:1,4-β-glucanase/lichenase
- Simple, convenient, rapid assay
- Well suited to automation
- Malt flour standard and lichenase standard included
(Bacillus subtilis)
(Bacillus subtilis)
