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Hexokinase/Glucose-6-phosphate dehydrogenase
Product code: E-HKGDH
€162.00
10 mL Hexokinase (420 U/mL) + G6P-DH (210 U/mL)
Prices exclude VAT
Content: | 10 mL Hexokinase (420 U/mL) + G6P-DH (210 U/mL) |
Shipping Temperature: | Ambient |
Storage Temperature: | 2-8oC |
Formulation: | In 3.2 M ammonium sulphate |
Physical Form: | Suspension |
Stability: | > 4 years at 4oC |
Enzyme Activity: | Kinase |
EC Number: | Hexokinase: 2.7.1.1 G6P-DH: 1.1.1.49 |
CAS Number: | Hexokinase: 9001-51-8 G6P-DH: 9001-40-5 |
Synonyms: | Hexokinase: Hexokinase; ATP:D-hexose 6-phosphotransferase G6P-DH: glucose-6-phosphate dehydrogenase (NADP+); D-glucose-6-phosphate:NADP+ 1-oxidoreductase |
Source: | Yeast |
Concentration: | Hexokinase: ~ 194 U/mL G6P-DH: ~ 665 U/mL |
Expression: | Hexokinase: Recombinant from yeast G6P-DH: Recombinant from Leuconostoc mesenteroides |
Specificity: | Hexokinase: Catalyses the reaction: ATP + D-hexose = ADP + D-hexose 6-phosphate Phopshorylates D-glucose, D-mannose, D-fructose, sorbitol and D-glucosamine. Glucose-6-phosphate dehydrogenase: Catalyses the reaction: D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+ |
Unit Definition: | Hexokinase: One Unit of hexokinase activity is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ in the presence of D-glucose and glucose-6-phosphate dehydrogenase at pH 7.5 and 25oC. G6P-DH: One Unit of Glucose-6-phosphate dehydrogenase activity is the amount of enzyme required to convert one μmole of glucose-6-phosphate to 6-phosphogluconate per minute, in the presence of NADP+ at pH 7.8 and 25oC. |
Temperature Optima: | 40oC |
pH Optima: | 7.4 |
Application examples: | Applications for the measurement of glucose and other hexoses in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries. |
High purity Hexokinase (yeast) / Glucose-6-phosphate dehydrogenase (G6P-DH) (Leuconostoc mesenteroides) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
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Associated Enzymes
E-FDHCB – Formate dehydrogenase (Candida boidinii) E-GALMUT – Galactose dehydrogenase – Galactose mutarotase