Antibiotic

Hygromycin B is an aminocyclitol antibiotic which specifically inhibits protein synthesis in both prokaryotes, eukaryotes and animal cells. Hygromycin B is used as a cell screening antibiotic of eukaryotic cells as well as prokaryotic cells. This selection market is used especially for transformation of plant cells.

[Specification of Hygromycin B]Solubility: Soluble in H₂O and methanol (10g/L).pH at 25C (25 g/L): 9.5~12.5Potency: min. 1,000 units/mg

[References]

1. Gritz, L. and Davies, J.: Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae, Gene, 25, 179-188 (1983).
2. Gonzales, R., et al.: Transformation of the Dermatophyte Trichophyton mentagrophytes to Hygromycin B Resistance, Infect. Immun., 57, 2923-2925 (1989).
3. Mohr, G.: Rapid detection of bacterial hygromycin B phosphotransferase in Aspergillus niger transformants, Appl. Microbiol. Biotechnol., 30, 371-374 (1989)

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