Description

TruePrime™ WGA kit uses a revolutionary novel multiple displacement amplification method based on the combination of the recently discovered DNA primase “TthPrimPol” and the extremely processive and high-fidelity Phi29 DNA polymerase to amplify uniformly total genomic DNA from purified material. The extraordinary strand displacement capacity of the Phi29 DNA polymerase allows TthPrimPol to generate new primers on the displaced strands that are extended by Phi29 DNA pol, resulting in exponential isothermal DNA amplification.

Supply

TruePrime™ WGA kit is supplied in a single box, with sufficient reagents for either 25 or 100 amplification reactions. The system consists of seven components, including the enzymes and buffers to perform the amplification reactions. Contents are provided in clear, screw-cap tubes with colour-coded lids as referenced in the TruePrime™ WGA kit handbook.

Shipping and Storage

TruePrime™ WGA kit is shipped on dry ice. Upon receipt, the kit should be stored immediately at -20°C in a non-frost-free (constant-temperature) freezer. When stored under these conditions and handled correctly, the products can be kept at least six months at -20ºC or one year at -80ºC without showing any reduction in performance.

Technical specifications

TruePrime™ WGA kit uses a novel and reliable method to achieve accurate genome amplification from purified material. Dedicated buffers and enzymes deliver microgram quantities of DNA. Typical DNA yields from a TruePrime™ WGA kit reaction are above 5 µg per 50 µl reaction when starting from 1 ng of genomic DNA. Yields and kinetics will vary if crude or un-quantified samples are amplified. Reactions without input DNA (no template controls) do not produce any amplification product during 3 hour reaction times. Mean product length is greater than 10 kb. Store amplified DNA at 4°C for short-term storage or -20°C for long-term storage.
TruePrime™ WGA kit uses alkaline incubation to allow DNA denaturation of genomic DNA with very low DNA fragmentation, resulting in amplified DNA with high integrity and maximizing the length of the amplification fragments, so that most of the sequences are uniformly represented.

Quality Control

Each batch of TruePrime™ WGA kit is tested against predetermined specifications to ensure consistent product quality. Enzymes used in the kit have been tested separately to ensure adherence to specifications.

Intended use

TruePrime™ WGA kit is intended for molecular biology use only and in vitro use only. This product is not intended for diagnosis, prevention or treatment of a disease in humans beings or animals.

Patent

Patent protection for TthPrimPol and methods associated with its use is pending.

Safety information:

Safety data sheets are available for download.

This material may contain substances or activities that are harmful to human health. It should not be ingested, inhaled, or brought into contact with skin, and handled with appropriate care in accordance with the principles of good laboratory practices.

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