The L-Asparagine/L-Glutamine/Ammonia test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-asparagine, L-glutamine and ammonia as acrylamide precursors in the food industry, or as cell culture media/supernatant components, or in other materials.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4^oC.

UV-method for the determination of L-Asparagine, L-Glutamine 
and Ammonia in potatoes, foodstuffs and cell culture media

Principle:
                               (glutaminase)
(1) L-Glutamine + H₂O → L-glutamate + NH₄⁺

                             (microbial glutamate dehydrogenase)
(2) NH₄⁺ + 2-oxoglutarate + NADPH → L-glutamate + NADP⁺ + H₂O

                                (asparaginase)
(3) L-Asparagine + H₂O → L-aspartate + NH₄⁺

Kit size:                              50 assays of each
Method:                              Spectrophotometric at 340 nm
Reaction time:                   ~ 20 min
Detection limit:                  0.50 mg/L (L-asparagine)
                                           0.54 mg/L (L-glutamine)
                                           0.06 mg/L (ammonia)
Application examples: 
Potatoes, potato products, vegetables, cereals and other materials
(e.g. biological cultures, samples, etc.)
Method recognition:          Novel method 

Advantages

• Very rapid reaction due to use of uninhibited glutamate dehydrogenase
   
• All enzymes supplied as stabilised suspensions
   
• Only kit available
   
• Very cost effective
   
• All reagents stable for > 2 years after preparation
   
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
   
• Standard included

K-ASNAM_DATA.pdf:
m.catalog.equl.cn.pdf:

Sample Pre-treatment Format: