每个套件包含大约184个测试样品加控制,包含2个微量滴定板。
 

Avian Reoviruses (REO) are thought to be responsible for viral arthritis, stunting syndrome, respiratory disease, enteric disease, malabsorption syndrome, and osteoporosis. REO has also been isolated from non-diseased chickens. The nature, symptoms, and severity of REO induced diseases varies with host age, virulence, and route of exposure1. Antibodies to avian viruses in serum can be detected by the Enzyme Linked Immunosorbant Assay (ELISA). The levels of REO antibodies in serum, as well as the immune status of entire flocks can be monitored using ELISA.
禽呼肠孤病毒（REO）被认为是引起病毒性关节炎，发育不良综合征，呼吸道疾病，肠道疾病，吸收不良综合征，与骨质疏松症。雷奥也已经从非病鸡隔离。自然，症状，和稀土诱导的疾病的严重程度随寄主龄期，毒力，和路径exposure1。血清中的禽流感病毒抗体可通过酶联免疫吸附试验（ELISA）检测。血清中REO抗体水平，以及整个鸡群免疫状态可监测使用ELISA。
Inactivated antigen is bound to a microwell. Diluted sera is added to the microwell and incubated. Any anti-REO antibodies present in the serum will bind to the REO on the microwell forming an REO antigen-antibody complex. After washing away any unbound material, anti-Chicken IgG Alkaline Phosphatase conjugate is added to the microwell. The conjugate will bind to the REO antigen-antibody complex. After incubation any unbound conjugate is removed by washing. Enzyme substrate is then added to the microwell. The substrate reacts with any Alkaline Phosphatase present and forms a yellow product. In the final step the reaction is stopped, fixing the color. The intensity of the color is measured photometrically at 405 – 410 nm.
灭活抗原绑定到微孔。稀释的血清加入微孔培养。任何抗-抗体存在于血清中，将绑定到的微孔形成抗原抗体复合物的稀土氧化物REO。洗去任何未结合的物质后，抗鸡IgG碱性磷酸酶缀合物加入到微孔。共轭将绑定到REO抗原抗体复合物。孵育后洗涤除去任何未结合的。酶的底物，然后添加到微孔。基板有碱性磷酸酶存在反应并形成黄色产物。在最后一步反应停止，固定的颜色。颜色的强度是在405 – 410 nm光度测量。