Hampton晶体删选试剂盒

Hampton晶体删选试剂

1.Screens 晶体删选试剂盒

产品名称 货号 用途备注
Index Kit HR2-144 用于生物大分子结晶
CrystalScreen Kit HR2-110 用于蛋白质,核酸水溶性小分子结晶
Crystal Screen 2 Kit HR2-112
Pegrx 1 HR2-082 用于生物大分子结晶
Pegrx 2 HR2-084 用于生物大分子结晶
Peg/Ion Screen Kit HR2-126 用于生物大分子结晶
Peg/Ion 2 Screen™ HR2-098 用于生物大分子结晶
Saltrx Kit HR2-108 用于生物大分子结晶
Membfac Kit HR2-114 用于膜蛋白和溶解度有限的样品结晶
Crystal Screen Lite HR2-128 用于蛋白质,缩氨酸,核酸的结晶
Natrix Kit HR2-116 用于核酸和蛋白核酸复合物的结晶
Crystal Screen Cyro Kit HR2-122 用于蛋白质,可溶性缩氨酸,核酸和水溶性小分子的初次低温结晶
Nucleic Acid Mini Screen Kit HR2-118 用于核酸片段的结晶
Low Ionic Strength Screen HR2-120 用于完整单抗,单抗片段以及在低离子浓度下溶解度较低的样品结晶
Silver Bullet & Silver Bullet Bio HR2-078 用于生物大分子的初次和二次结晶
HR2-080
Peg/Tacsimate Ph5.8 Crystallization Reagent HR2-090
Peg/Tacsimate Ph 6.8 Crystallization Reagent HR2-092
Peg/Tacsimate Ph 7.8 Crystallization Reagent HR2-094
Additive Screen™ HR2-428 利用样品间和样品溶剂间相互作用来优化晶体生长和改变样品溶解度
产品名称 货号 用途备注
Detergent Screen 1 HR2-410 改善疏水作用引起的非特异性聚集
Detergent Screen 2 HR2-411
Detergent Screen 3 HR2-412
Detergent Screen HR2-408
Heavy Atom Screen Pt HR2-442 Heavy Atoms For Multiple Isomorphous Replacement
Heavy Atom Screen Au HR2-444
Heavy Atom Screen Hg HR2-446
Heavy Atom Screen M1 HR2-448
Heavy Atom Screen M2 HR2-450
I3c Phasing Kit HR3-133

2.Custom Shop Crystallization Reagents 结晶试剂盒中单独的试剂

  1. Optimize™ Reagents晶体生长条件优化试剂

⑴ Optimize™ – Polymers聚合体

⑵ Optimize™- Organics (Volatile) 挥发性有机物

⑶ Optimize™- Organics (Non-Volatile)非挥发性的有机物

⑷ Optimize™- Salts 盐类

⑸ Optimize™- Buffers缓冲液

⑹ Optimize™- Solubilizing Agents (NDSB) 溶解试剂NDSB

⑺ Optimize™ – Reducing Agent还原剂-TCEP氢氯化物

⑻ Optimize™ – Cryoprotectants

⑼ Optimize™ – Oils用于结晶的油类

⑽ Optimize™ – Silica Hydrogel硅胶

⑾ Optimize™ – LM Agarose低熔点琼脂糖
5.Crystallization Plates, Hardware & Accessories结晶耗材
⑴ 24 Well Crystallization Plates 24孔晶体板-分悬滴和坐滴两种
⑵ 48 Well Crystallization Plates 48孔晶体板
⑶ 72 Well Crystallization Plates72孔晶体板
⑷ 96 Well Crystallization Plates 96孔晶体板等

Hampton 膜蛋白结晶试剂 MembFac • Crystal Screen Lite • MembFac HT

Hampton  MembFac • Crystal Screen Lite • MembFac HT

Applications

Primary sparse matrix crystallization screen for membrane proteins and samples with limited solubility   膜蛋白和样品溶解度有限样品

Features

  Membrane protein sparse matrix screens

  Sparse matrix formula efficiently samples salts, polymers, organics and pH

  pH range 4.6 – 8.5

  Formulated for use with detergents

   Tube or Deep Well block format

   Crystal Screen Lite features a lower ionic strength than the original Crystal Screen

Description
MembFac is a highly effective sparse matrix screen specifically designed as a preliminary screen for the crystallization of membrane proteins. MembFac is based upon the highly effective biased sparse matrix methodology. The MembFac screen matrix is optimized for the crystallization of membrane proteins.

MembFac contains 48 unique reagent formulations. With this set of 48 conditions, numerous chemicals and chemical combinations are utilized, allowing one to evaluate a large variety of potential crystallization conditions. MembFac covers a broad pH range between 4 and 9.

The Rationale
The basic rationale of MembFac is to perform a sparse matrix screen while changing the detergent dimension because detergents play an important factor in the crystallization of membrane proteins. A MembFac screen is to be completed for each detergent. Detergent screens are available separately.

The Method
The membrane protein of interest is first isolated in the detergent which gives the highest stability and activity. The protein concentration should be approximately 10 to 20 mg/ml and the detergent concentration should be only slightly above the CMC. The sample is then set against MembFac, using all 48 solutions plus the screening detergent(s) of choice. The screening detergent concentration should be approximately one to three times the CMC. Hence, for each detergent screened, one would utilize approximately 1 to 2 mg of sample.

The MembFac protocol was used to crystallize membrane proteins including SQR, fumerate: quinone oxireductase, LHII, and LHCII.

Crystal Screen Lite is a sparse matrix of trial crystallization reagent conditions based upon the Crystal Screen™.3 The primary screen variables are salt, pH, and precipitant (salts, polymers, volatile organics, and non-volatile organics).2Crystal Screen Lite differs from the original Crystal Screen kit in that the primary precipitant reagents are one-half the concentration of that used in the original Crystal Screen formulation. The secondary salts, ions, and buffers remain at the original Crystal Screen concentration. Reducing the primary concentration of the primary precipitant results in a screen which is “more gentle” on the sample and typically produces much less precipitate conditions than the original Crystal Screen.

MembFac contains 48 unique reagents, 10 ml each.

MembFac HT contains 1 ml each of all 48 reagents from MembFac and reagents 1-48 from Crystal Screen Lite in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

CAT NO NAME DESCRIPTION
HR2-114 MembFac 10 ml, tube format
HR2-128 Crystal Screen Lite 10 ml, tube format
HR2-137 MembFac HT 1 ml, Deep Well block format

Hampton AlumaSeal™ II Sealing Film and Applicator

Hampton AlumaSeal™ II Sealing Film and Applicator

Applications

Sealing film used to reseal HT format screen kits in polypropylene blocks and plates

Features

Excellent seal
Film conforms to raised chimney wells
Easily pierceable with single or multichannel pipettors and robotic probes
Heat & cold resistant, recommended for temperatures from -80 °C to +120 °C
Certified DNase-, RNase-, and nucleic-acid-free
Less evaporation than clear films
Excellent barrier properties, virtually no reagent evaporation or drying
 Description
A 38 µm soft non-permeable aluminum foil sealing film with strong medical-grade adhesive, AlumaSeal II sealing films eliminate the need for heat-sealing devices or mats during the resealing of reagents in polypropylene deep well blocks. Each sealing film measures 82.6 x 142.9 mm and offers sufficient sealing area for 96 deep well blocks. Length between the perforations with end tabs removed is 125.4 mm. Compared to other aluminum foils, AlumaSeal II has less tendency to roll back on itself when removing the backing paper and it conforms well to the plate during application.AlumaSeal II is a soft, pierceable adhesive film designed for the convenient and rapid sealing of polypropylene deep well blocks. A multiple split backing with two end tabs allows for easy, accurate positioning and secure sealing. The use of an adhesive sealing film minimizes evaporation and helps to prevent well-to-well cross contamination in reagent blocks. AlumaSeal II films are easily pierced by pipettte tips or robotic probes or piercing tools for direct reagent recovery without significant gumming by adhesive.

AlumaSeal™ II Sealing Film and Applicator

AlumaSeal™ II Sealing Film and Applicator

CAT NO NAME DESCRIPTION
HR8-069 AlumaSeal II Sealing Film 100 pack
HR4-413 Film Sealing Paddle 5 pack

Hampton热销产品

Hampton Research是 美国最权威的蛋白结晶试剂盒提供商,产品齐全,性价比高。

在生命科学领域,上海金畔生物为业界提供了丰富的实验室和生物医药生产研发的产品。庆祝上海金畔生物正式成为Hampton的签约代理商,在这里要感谢广大客户多年来对上海金畔生物科技有限公司的支持和厚爱,我们将一如既往的为广大客户带来Hampton高品质的产品和服务,欢迎广大新老客户来电咨询。‘

美国Hampton Research公司位于加利福尼亚州,是一家专门从事蛋白质晶体研究的生产商。该公司向全球科研人员提供全面的晶体研究试剂和相关实验耗材,同时提供晶体设计和合成方面的定制服务。Hampton Research公司以其先进的生物大分子结晶技术和广泛实用的产品线,不仅为全世界晶体研究人员的工作带来了便捷,而且也受益于科研人员的回馈不断发展自己,目前已成为晶体研究领域最受信赖的品牌之一。

Hampton research主要产品:

Crystallization Screens 用于筛选和优化蛋白质/多肽/核酸结晶的一系列经验证的高精配方型溶液

Optimize Reagents 结晶级别盐类,聚合物,有机溶液或缓冲液

Crystallization Plates, Hardware & Accessories 结晶板(悬滴法和坐滴法,微量透析,微配液,自由界面扩散,凝胶以及无容器悬浮法)及其他配件

Cryocrystallography 低温晶体学耗材如 Crystalcap system,cryoloops

Goniometer Heads&Supplies 测角器头和配件

Protein Crystallization Standards 结晶级别标准蛋白

Custom Shop Crystallization Reagents 定制试剂盒和结晶溶液内的单一组分

StockOptions Kits 内含盐类,缓冲液和冷冻液的简便型试剂盒

Tools,Seeding&Resin 结晶用探针,结晶笔,seeding法结晶工具

Capillary Mounts & Supplies 毛细管,玻璃纤维, 蜡,黏合剂和密封剂

Hampton热销产品

  • StockOptions pH Buffer Kit HR2-241

    Crystallization grade buffer stocks for crystal screening, optimization, & production

    Crystal Screen Cryo HR2-122

    Primary screen with cryo for proteins, soluble peptides, nucleic acids, & water soluble small molecules

  • Natrix HR2-116

    Primary biased sparse matrix crystallization screen for nucleic acids & protein/nucleic acid complexes

  • MembFac HR2-114

    Primary sparse matrix crystallization screen for membrane proteins and samples with limited solubility

  • SaltRx HR2-136

    Primary or secondary, salt and pH matrix crystallization screen for biological macromolecules

  • Grid Screens HR2-248

    Primary or secondary, salt, polymer, organic and pH grid crystallization screen for biological macromolecules

  • PEG/Ion HR2-126

    Primary or secondary, polymer, salt and pH matrix crystallization screen for biological macromolecules

  • PCT™ Pre-Crystallization Test HR2-140

    Determine the appropriate sample concentration for crystallization screening

  • Index HR2-144

    Primary, diverse reagent system crystallization screen for proteins, complexes, peptides, nucleic acids, & water soluble small molecules

  • Crystal Screen HR2-110

    Primary screen for proteins, soluble peptides, nucleic acids, & water soluble small molecules;Sparse matrix additive screen

  • PEGRx HR2-082

    Primary and secondary, polymer and pH based crystallization screen for biological macromolecules

  • Additive Screen HR2-428

    Manipulate sample-sample & sample-solvent interactions to improve crystals or alter sample solubility

  • Detergent Screen HR2-408

    Prevent and manipulate non-specific aggregation due to hydrophobic interactions

  • CryoPro HR2-073

    Water soluble cryoprotectant reagent set designed for the cryopreservation of biological macromolecular crystals

  • StockOptions Salt HR2-245

    Crystallization grade salt reagent stock solutions for screen formulation and optimization

  • Siliconized Glass Cover Slides HR3-217

    Hanging, sitting or sandwich drop crystallization

  • Intelli-Plate 96 HR3-185

    蛋白结晶座滴板

  • CrystalCap HR4-733
  • CryoLoop HR4-623

    • CryoTong™ – Long HR5-104

      Crystal transfer under cryo temperature

    • CrystalWand™ Magnetic HR4-729

      CrystalCap Magnetic & CrystalCap Copper Magnetic handling tool

    • Vial Clamp™ – Straight HR4-670

      Vial support and manipulation

    • CrystalCap™ Holder HR4-707
    • CryoCane HR4-709

      Organized storage of CrystalCaps