Hampton GRAS 3 • GRAS 4 Screens

Hampton GRAS 3 • GRAS 4 Screens

Applications

a.GRAS reagent crystallization screen for proteins, including monoclonal antibodies, where Salt is the primary, and Polyethylene glycol the secondary reagent, sampling pH 4-9

b.Identify GRAS based reagents that promote crystallization of biotherapeutics for bioprocess, bioformulation, and continuous flow manufacturing applications

Features

Developed at Hampton Research
Generally Recognized As Safe reagent formulation
Samples pH 4.0 – 9.0 without an added buffer
Primary crystallization reagent:

24 unique Salts

 

Secondary crystallization reagent:

GRAS Screen 3: PEG 300, 400, MME 550, & 600
GRAS Screen 4: PEG 1,000, MME 2,000, 3,350 & 4,000

 

Compatible with Vapor diffusion, microbatch, free interface diffusion
Reagents soluble between 4°C and 30°C

 Description
GRAS Screen™ 3 and GRAS Screen™ 4 were developed by Hampton Research for the crystallization of proteins, including monoclonal antibodies. Each of the chemicals in GRAS Screen 3 and 4 have been used under one or more of the following categories. As (1) a Generally Recognized As Safe (GRAS) substance, (2) a pharmaceutical excipient, (3) a normal physiological constituent, (4) a metabolic byproduct, and/or (5) a Everything Added to Food in the United States (EAFUS) substance.

GRAS Screen 3 samples twenty four salts, encompassing pH 4-9 versus four low molecular weight Polyethylene glycols (300, 400, MME 550, & 600). GRAS Screen 3 is supplied in a 96 Deep Well block format and is compatible with robotic and multi-channel pipet liquid handling systems. GRAS Screen 3 is compatible with vapor diffusion, free interface diffusion, and microbatch crystallization methods. For research use only.

GRAS Screen 4 samples twenty four salts, encompassing pH 4-9 versus four medium molecular weight Polyethylene glycols (1,000, MME 2,000, 3,350 & 4,000). GRAS Screen 4 is supplied in a 96 Deep Well block format and is compatible with robotic and multi-channel pipet liquid handling systems. GRAS Screen 4 is compatible with vapor diffusion, free interface diffusion, and microbatch crystallization methods. For research use only.

 

CAT NO NAME DESCRIPTION
HR2-453 GRAS Screen 3 1 ml, Deep Well block format
HR2-454 GRAS Screen 4 1 ml, Deep Well block format

低温泡沫杜瓦瓶 Spearlab Cryogenic Foam Dewars

低温泡沫杜瓦瓶 Spearlab Cryogenic Foam Dewars
Spearlab Cryogenic Foam Dewars
Spearlab Dewars
Spearlab Cryogenic Foam Dewars
HR4-662 Cryogenic Foam Dewar (500 ml) – Small  500ml规格
Spearlab Cryogenic Foam Dewars
HR4-675 – Spearlab Cryogenic Foam Dewar (2 liter)  2L规格
低温泡沫杜瓦瓶 Spearlab Cryogenic Foam Dewars

Applications 应用

    Cryocrystallography 蛋白结晶学

Features

  Each dewar is supplied with a lid  每个杜瓦瓶都配有盖子

  Proprietary foam construction (USPTO # 7,971,744)

  Mini (130 ml), Small (500 ml), Standard (800 ml), Large (1400 ml) and Tall (1800 ml)

   Reduced ice formation on dewar lip

  Easier to dry

   Foam construction reduces slipping

Description
Spearlab FD-130 Cryogenic Foam Dewar (130 ml) – Mini : Small, hexagonal outside, hand held foam dewar, maximum volume 175 ml. Each dewar is supplied with a matching foam lid to insulate the contents from ambient air. Dimensions of the cylindrical cavity in this vessel are 2 inches in diameter by 3.5 inches deep, so that it easily holds 130 ml and a maximum volume of 175 ml of liquid nitrogen. The HR4-676 has an overall height of approximately 4.5 inches without cover and the width is 3.5 inches at the top and 3.75 inches on the bottom (measured on the flats of the hexagon). Circular cover is approximately 0.5 inches tall x 3.5 inches diameter.

Spearlab Cryogenic Foam Dewar (500 ml) – Small: The small foam dewar shape is circular, with a protruding handle, so that it resembles a large teardrop. Each dewar is supplied with a matching foam lid to insulate the contents from ambient air. Dimensions of the cylindrical cavity in this vessel are 4.5 inches in diameter by 2.8 inches deep, so that it easily holds 500 ml of liquid nitrogen.

Spearlab FD-800 Cryogenic Foam Dewar (800 ml) – Standard: The standard foam dewar shape is circular, with a protruding handle, so that it resembles a large teardrop. Each dewar is supplied with a matching foam lid to insulate the contents from ambient air. Dimensions of the cylindrical cavity in this vessel are 5.8 inches in diameter by 2.8 inches deep, so that it easily holds 800 ml of liquid nitrogen.

Spearlab FD-1400 Cryogenic Foam Dewar (1400 ml) – Large: The large foam dewar shape is circular, with a protruding handle, so that it resembles a large teardrop. Each dewar is supplied with a matching foam lid to insulate the contents from ambient air. Dimensions of the cylindrical cavity in this vessel are 6.3 inches in diameter by 3.8 inches deep, so that it easily holds 1400 ml of liquid nitrogen. Overall height is approximately 6 inches without cover and the width is 9 inches on the short axis and 11 inches on the long axis. Cover approximately 0.5 inches tall x 9 inches diameter.

Spearlab FD-1800 Cryogenic Foam Dewar (1800 ml) – Tall: The tall foam dewar shape is a tapered octagon on the outside with a cylindrical interior. The tapered octagon features a wide, stable base. Each dewar is supplied with a matching foam lid to insulate the contents from ambient air. Dimensions of the cylindrical cavity in this vessel are 3.5 inches in diameter by 12.5 inches deep, so that it holds approximately 1,800 ml of liquid nitrogen. The outside dimensions of the tall dewar are 15.25 inches tall with a 6.25 inches wide top and a 7.75 inches wide base (measured on the flats of the octagon). The cover is 6.5 inches in diameter and 0.5 inches thick.

The patented (USPTO # 7,971,744) design of the Spearlab Cryogenic Foam Dewar makes it easy to handle and safer to use than a traditional low profile glass dewar. Also, because of its lower thermal mass, a foam vessel will cause less boil off when it is filled with liquid nitrogen. Additionally, the dewar will accumulate less frost during regular use. The end result is that less liquid nitrogen is consumed.

The dewars are made from cross-linked polyethylene foam. A density of 4 pounds per cubic foot is used for the 130 ml and 800 ml dewars. A density of 2 pounds per foot is used for the 1400 ml and 2 liter dewars. The higher density foam has significantly higher mechanical strength and stiffness. The lower density foam has slightly lower thermal conductivity. Like most lightweight insulation materials, the thermal conductivity of the foam is predominantly determined by the thermal conductivity of air, which eventually permeates the pores of the foam material.

CAT NO NAME DESCRIPTION
HR4-676 Cryogenic Foam Dewar (130 ml) – Mini each
HR4-662 Cryogenic Foam Dewar (500 ml) – Small each
HR4-673 Cryogenic Foam Dewar (800 ml) – Standard each
HR4-674 Cryogenic Foam Dewar (1400 ml) – Large each
HR4-675 Cryogenic Foam Dewar (2 liter) – Tall each

Hampton 蛋白结晶向导管Wizard Tube

Wizard Tube 
The Wizard™ Classic line of random sparse matrix screens is designed to increase your probability of producing crystals during the coarse screening phase when crystallizing biological macromolecules (proteins, nucleic acids, peptides, and combinations thereof).
The Wizard Classic reagents are proven to be a highly effective starting point in the screening of biological macromolecules. The Wizard Classic formulations include a large range of crystallants, buffers, and salts covering a broad range of crystallization space at pH levels from pH 4.5 to pH 10.5.Choose from Wizard Classic 1, 2, 3, or 4 non-overlapping formulations in matrix blocks or tubes.

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Wizard Cubic LCP
The tools in the Wizard Cubic LCP (lipidic cubic phase) Kit enable researchers to prepare LCP-type crystallizations by hand. Ideal for low-protein experiments: effective protein volume for a single crystallization experiment is about 80 nanoliters. Wizard Cubic LCP Kit tools work especially well when traditional methods have failed to yield crystals. Lipidic cubic phase has worked well for the crystallization of 7TM membrane proteins (proteins with seven transmembrane helices). Four out of six GPCRs (G-protein coupled receptors), an important membrane protein class, and several microbial 7TM proteins have been crystallized using the LCP approach.

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CRYO (I、II)
The Wizard Cryo™ line of random sparse matrix screens is designed for scientists who want to avoid the additional step of optimizing a cryoprotectant condition. Every Wizard Cryo formulation flash-freezes to a clear, amorphous glass in liquid nitrogen or in a cryo-stream at 100K. Crystals can be frozen directly from their growth drops, avoiding the additional step of pre-equilibration with an artificial cryo-condition that can damage the crystal. Eleven different cryocrystallants and sparing use of glycerol ensures a broad sampling of possible cryo conditions. Choose from Wizard Cryo 1 or 2 formulations in tubes or Wizard Cryo 1 and 2 together in a 96-well matrix block.

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Hampton CrystalEX Second Generation (Corning)

Hampton CrystalEX Second Generation (Corning)

Crystal<i>EX</i> Second Generation (Corning)

CrystalEx Plates

Applications

   Sitting drop crystallization

Features

96 well sitting drop plate
Round and conical flat bottom drop well shapes
1, 2 or 4 microliter drop wells
1, 3 or 5 drop wells per reservoir
Hydrophilicity treated or untreated
PZero or COC plate material–UV imaging compatible

Description

The second generation of Corning® 96 Well, sitting drop format plates are built to SBS specifications, making them well suited for high throughput crystallization and are fully compatible with robotic equipment. The plates are available in several different configurations with varying drop well shapes, plate materials, and number of drop wells per reagent well. The basic plate design is one reagent well flanked by one or three drop wells, with SBS standard spacing between the centers of adjacent well clusters. One may choose a plate with small (1 µl), medium (2 µl), or large (4 µl) drop well volumes. The choice of round or conical flat well shapes are available. The PZero polymer provides for zero background polarization and is non-birefringent. PZero plates are not treated. The COC polymer offers high chemical resistance. Both types of plastic feature improved transparency. The reservoir numbers are embossed on each individual well for easy identification. Drop well locations conform to SBS standards for robotic handling. The low-volume reagent well saves on reagent costs. The plates can be sealed using Crystal Clear Sealing Film (HR3-609), 3 inch wide Crystal Clear Sealing Tape (HR4-506) or ClearSeal Film™ (HR4-521).

Corning has discontinued 3557 – special pricing and limited inventory available for this item only.

CAT NO NAME DESCRIPTION
HR8-135 Corning 3556 4µl round drop well, 1 drop well, COC, untreated – 10 plate case
HR8-134 Corning 3556 4 µl  round drop well, 1 drop well, COC, untreated – 40 plate case
HR8-137 Corning 3551 4 µl conical flat drop well, 1 drop well, COC, treated – 10 plate case
HR8-136 Corning 3551 4µl conical flat drop well, 1 drop well, COC, treated – 40 plate case
HR8-139 Corning 3552 2 µl round drop well, 3 drop well, COC, untreated – 10 plate case
HR8-138 Corning 3552 2µl  round drop well, 3 drop well, COC, untreated – 40 plate case
HR8-141 Corning 3553 2 µl conical flat drop well, 3 drop well, COC, untreated – 10 plate case
HR8-140 Corning 3553 2 µl conical flat drop well, 3 drop well, COC, untreated – 40 plate case
HR8-147 Corning 3550 1µll conical flat drop well, 3 drop well, COC, untreated – 10 plate case
HR8-146 Corning 3550 1µl conical flat drop well, 3 drop well, COC, untreated – 40 plate case
HR8-160 Corning 3557 1 µl conical flat drop well, 5 drop well, PZero – 10 plate case
HR8-158 Corning 3557 1 µl conical flat dro p well, 5 drop well, PZero – 40 plate case

Hampton PEGRx 聚乙二醇结晶试剂盒

Hampton PEGRx

PEGRx 1 • PEGRx 2 • PEGRx HT

Applications   主要是调节样本的PH值

  Primary and secondary, polymer and pH based crystallization screen for biological macromolecules

PEGRx is a primary and secondary, polymer and pH based crystallization screen developed at Hampton Research. It is designed to evaluate polymer based crystallization reagents and pH in low (PEGRx 1) to medium ionic strength (PEGRx 2). It is also designed for use as a secondary or optimization screen to follow the Hampton Research Index screen when polymer based reagents produce hits and interesting solubility leads.PEGRx 1 is a crystallization reagent kit designed to evaluate an array of polymers of varying molecular weight in a low ionic strength environment versus a wide range of pH. Polymer reagents include Polyethylene glycols, Polyethylene glycol monomethyl ethers, and Jeffamines®. The molecular weight range between 200 and 20,000 is evaluated in a low ionic strength formulation. Ten different buffers are used to span the range of pH between 3.5 and 9. The primary screen variables are polymer type, polymer molecular weight, pH and low ionic strength.

PEGRx 2 is a crystallization reagent kit designed to evaluate an array of polymers of varying molecular weight in a medium ionic strength environment in the presence of additives, salts, volatile organics and polyols versus a wide range of pH. Polymer reagents include Polyethylene glycols and Polyethylene glycol monomethyl ethers. The polymer molecular weight range between 200 and 20,000 is evaluated in a medium ionic strength formulation. Ten different buffers are used to span the range of pH between 3.5 and 9. The primary screen variables are polymer type, polymer molecular weight, pH and secondary reagents which include additives, salts, volatile organics and polyols.上海金畔生物作为Hampton蛋白结晶产品的代理商,竭诚为您服务,欢迎新老客户咨询。PEGRx 1 contains 48 unique reagents, 10 ml each.

PEGRx 2 contains 48 unique reagents, 10 ml each

PEGRx HT contains the 96 PEGRx 1 and PEGRx 2 reagent in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

PEGRx 1 • PEGRx 2 • PEGRx HT

PEGRx

PEGRx 1 • PEGRx 2 • PEGRx HT

DesR crystals grown using Hampton Research PEGRx, Jin Ho Moon, Korea University.

CAT NO NAME DESCRIPTION
HR2-082 PEGRx 1 10 ml, tube format
HR2-084 PEGRx 2 10 ml, tube format
HR2-086 PEGRx HT 1 ml, Deep Well block format