MultiBac™,MultiBacMam™和SynBac™感受态细胞试剂盒 Geneva Biotech to manufacture and distribute MultiBac™,MultiBacMam™, and SynBac™ Cell Lines and Kits 货号:GENEVA-BIOTECH 品牌:


MultiBac™,MultiBacMam™和SynBac™感受态细胞试剂盒

英文名:Geneva Biotech to manufacture and distribute MultiBac™,MultiBacMam™, and SynBac™ Cell Lines and Kits

货号:GENEVA-BIOTECH

报价:价格面议

商品描述

 MultiBac™,MultiBacMam™和SynBac™感受态细胞试剂盒

 
MultiBac™
MultiBac™表达系统使用DNA重组,可以轻松,高通量地构建杆状病毒载体,用于昆虫细胞中多蛋白的表达。需要多蛋白共表达来产生蛋白质复合物,例如多价疫苗,或在同一细胞中产生多个单个蛋白质,例如用于信号传导途径。多表达技术也是新兴合成生物学革命的基石。 MultiBac™平台已用于生成完整的完整信号转导途径,如GPCR信号级联,激酶级联和蛋白酶级联。 MultiBac™也已用于生产病毒样颗粒(VLPs)和多种多蛋白复合物。诸如MultiBac TM的杆状病毒表达载体系统(BEVS)是用于产生用于药物发现的蛋白激酶,GPCR和离子通道的前线技术平台,并且是作为基因治疗的递送载体的主要关注领域。使用杆状病毒作为载体的昆虫细胞表达也越来越多地用于生物制药的制造,最近出现了显着的成功案例,包括Cervarix®和Flublok®。

MultiBacMam™
BacMam™是一种日益流行的系统,可促进异源DNA向广泛的哺乳动物转导靶标的传递。 BacMam使用杆状病毒作为载体,与其他哺乳动物转染方法相比具有明显的优势,因为杆状病毒具有大的异源DNA携带能力(> 40kbp),广泛的哺乳动物细胞类型对杆状病毒的摄取,以及复杂的分子生物学技术在过去的20年中,我们已经进化出来支持杆状病毒表达载体的产生。 MultiBacMam™是第一个专门设计用于基于模块化,基于DNA重组的多表达盒组装的BacMam系统,用于在哺乳动物细胞中多蛋白共表达。 MultiBacMam™使用与MultiBac™相同的DNA重组技术,但使用哺乳动物启动子和“哺乳动物”杆状病毒 – 在杆状病毒表面展示VSV肽,使病毒摄取增加一个数量级,并使用稳定整合的荧光蛋白简化监测实验。 MultiBacMam™转移载体是完全合成的,仅包含功能性DNA,因此体积小且易于操作。 MultiBacMam™杆状病毒基因组(DH10EMBacVSV)正在不断发展,以去除有害/非必需基因(例如v-cath和chiA)和DNA重组热点,并含有整合的荧光蛋白表达盒,以便于监测病毒滴度。与先前的BacMam病毒系统相比,MultiBacMam™病毒颗粒在其表面上显示VSV肽以提高进入哺乳动物细胞的效率约1个数量级。
 
SynBac™
使用杆状病毒的传统工艺规模扩大是众所周知的问题,在扩大规模期间产量逐渐下降。 SynBac™是使用现代合成生物学技术创建的最小化的杆状病毒基因组。 SynBac™项目旨在通过创建显示出卓越DNA稳定性的最小杆状病毒基因组来改变药物靶标的发酵罐级杆状病毒生产,以及生产生物制药的规模生产。除了改善DNA稳定性外,我们最小化的基因组构成了需要向哺乳动物细胞,原代细胞或器官递送大量DNA货物的应用的起点。
 
订购产品(病毒基因组的商业用户另外需要需要商业许可)

以下产品瑞士工厂出厂价USD980:
DH10MultiBac 感受态细胞(12×100μl)
DH10EMBacY感受态细胞(12×100μl) 
DH10EMBacVSV感受态细胞(12×100μl)
PirHC感受态细胞(12×100μl) 
SynBac™感受态细胞(12×100μl)

以下产品瑞士工厂出厂价USD1480:
带有感受态细胞的完整MultiBac™试剂盒
带有感受态细胞的完整MultiBacMam™试剂盒
 

ZYMO DNASE I修饰酶 RESEARCH DNase I Set ZYMO RESEARCH DNase I Set 货号:E1009 品牌:


ZYMO DNASE I修饰酶 RESEARCH DNase I Set

英文名:ZYMO RESEARCH DNase I Set

货号:E1009

规格:1 Set

品牌: Jinpan

优惠价:3900.00 ( 原价4500.00 )

商品描述

DNase I cuts both double-stranded and single-stranded DNA, producing 3′-OH oligonucleotides. It is typically used for selectively degrading DNA in the presence of RNA. This DNase is suited for applications such as nick translation, production of random fragments, cleavage of genomic DNA for footprinting, removal of DNA template after in vitro transcription, and removal of DNA from RNA samples prior to applications such as RT-PCR. It is compatible with all of our RNA kits featuring in-column DNase digestion.

R&D 人来源重组 Recombinant Human IL-7, CF (抗体) Recombinant Human IL-7, CF 货号:207-IL-025/CF 品牌:R&D RD


R&D 人来源重组 Recombinant Human IL-7, CF (抗体)

英文名:Recombinant Human IL-7, CF

货号:207-IL-025/CF

规格:25ug

品牌: R&D RD

优惠价:4000.00 ( 原价6000.00 )

商品描述

Source:E. coli-derived, Asp26-His177, with an N-terminal Met
Accession #:P13232
N-terminal Sequence Analysis:Met
Purity:>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level:< 1.0 EU per 1 μg of the protein by the LAL method.
Predicted Molecular Mass:17 kDa
Activity:Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894.
The ED50 for this effect is typically 0.1-0.5 ng/mL.
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increase shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
Which formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Formulation: Lyophilized from a 0.2 µm filtered solution in PBS. See Certificate of Analysis for details.
Reconstitution: Reconstitute 5 µg vials at 50 µg/mL in sterile PBS. Reconstitute 25 µg or larger vials at 100 µg/mL in sterile PBS.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.

Beta葡聚糖[大麦;中黏度]5g ?-Glucan (Barley;Med Viscosity) 货号:MP-BGBM 品牌:Megazyme


Beta葡聚糖[大麦;中黏度]5g

英文名:?-Glucan (Barley;Med Viscosity)

货号:MP-BGBM

规格:5 grams

品牌: Megazyme

报价:4900.00

商品描述

Content: 5 g
Shipping Temperature: Ambient
Storage Temperature: Ambient
Physical Form: Powder
Stability: > 10 years under recommended storage conditions

 

高纯度β-Glucan(大麦;介质粘度)用于研究、生化酶化验和体外诊断分析。介质粘度β-glucan从大麦面粉。麦芽β-glucanase和多种纤维素酶的测定。适合酿造研究所测定粘度的测定。

High purity β-Glucan (Barley; Medium Viscosity) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Medium viscosity β-glucan from barley flour. For the assay of malt β-glucanase and cellulases. Suitable for Institute of Brewing viscometric assay.

 

CAS Number: 9041-22-9
Source: Barley flour
Molecular Weight: 251,000
Purity: ~ 95%
Viscosity: 20-30 cSt
Monosaccharides (%): Glucose = 95
Main Chain Glycosidic Linkage: β-1,4 and β-1,3
Substrate For (Enzyme): β-Glucanase/Lichenase
 

Fibronectin, Human, Lyophilized 5.0 mg Fibronectin, Human, Lyophilized 5.0 mg 货号:BM-5080-5MG 品牌:Advanced Biomatrix


Fibronectin, Human, Lyophilized 5.0 mg

英文名:Fibronectin, Human, Lyophilized 5.0 mg

货号:BM-5080-5MG

规格:5mg

品牌: Advanced Biomatrix

报价:5500.00

商品描述

Fibronectin is a widely used broad range natural cell attachment factor. This product has been purified from human plasma where it is found as a dimer and has a size of 440-500 kDa with two similar subunits (220-250 kDa ) linked by two disulfide bonds.

Fibronectin’s primary function is related to cell adhesion and attachment to extracellular matrix. Certain domains of Fibronectin have been found to play important roles that involve interactions with collagen, heparin and other cell surface glycosaminoglycans (GAGs).

Fibronectin is ideal for coating of surfaces and is typically used at a concentration  of 1-5 µg/cm2 . It is provided in user-friendly packaging for use and storage. Fibronectin is sterile filtered and is supplied as a sterile, lyophilized product.

Parameter, Testing, and Method

Fibronectin
Catalog # 5080

Form

Lyophilized Powder

Package Size

5 mg

Sterilization Method

0.2 micron filtration

Purity

>95%
as measured by HPLC

Formulation

1.12 mg glycine and0.058 mg sodium
chloride per mg of fibronectin

Source

Human, Plasma

Storage Temperature

2 to 10 °C prior to reconstitution
-20°C after reconstitution

Shelf Life

24 months

Shelf Life after Reconstitution

3 months

Cell Attachment Activity

Passes

Sterility

No Growth

Safety

Source material found negative for
infectious agents

 

 

 

 

References Downloads

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Clodrosome 荧光脱氧核糖核酸巨噬细胞清除试剂盒 Fluorescent-DiD Macrophage Depletion Kit 货号:8904-2ml 品牌:Encapsula NanoSciences


Clodrosome 荧光脱氧核糖核酸巨噬细胞清除试剂盒

英文名:Fluorescent-DiD Macrophage Depletion Kit

货号:8904-2ml

规格:2 mL

品牌: Encapsula NanoSciences

报价:3900.00

商品描述

Description
 
 
Macrophage depletion kits are composed of two vials; one vial of Clodrosome® (Clodronate liposomes) and one vial of Encapsome® (control liposomes containing no drug). The volume of the macrophage depletion kit represents the volume of each reagent individually. For example, 5 ml of macrophage depletion kit means 5 ml of Clodrosome® and 5 ml of Encapsome®. Each reagent in the kit can also be purchased individually.
 
Clodrosome® is a multilamellar liposome suspension in which clodronate is encapsulated in the aqueous compartments of the liposomes. Encapsome® is formulated and prepared identically to Clodrosome® except that clodronate is not added to the liposomes. The liposomes are filtered through 2 μm polycarbonate membranes to ensure that larger particles, which may be toxic to animals, are removed from the suspension. Both are prepared and packaged under sterile conditions. When animals or cells are treated with Clodrosome®, phagocytic cells recognize the liposomes as invading foreign particles and proceed to remove the liposomes from the local tissue or serum via phagocytosis. The liposomes then release clodronate into the cytosol resulting in cell death. Non-encapsulated clodronate cannot cross the cell membrane to initiate cell death.
 
Control liposomes (Encapsome®) are recognized and phagocytosed by the same mechanism as Clodrosome®. Since the control liposomes do not contain clodronate, the phagocytic cells are not killed. However, phagocytes do respond to the ingestion of control liposomes by cytokine secretion, temporary suspension of phagocytic activity and other responses described in the literature.
 
m-Clodrosome® and m-Encapsome® are mannosylated reagents that are specifically formulated to efficiently target the macrophages in central nervous systems and macrophages that contain more mannose receptors. For more information about these reagent see here.
 
Fluorescent liposomes (Fluoroliposome®) suitable for macrophage targeting and tracking are available. They can contain five different fluorescent dyes (DiA, DiD, DiI, DiO and DiR), which covers the entire spectrum. Fluorescent liposomes come in standard and mannosylated form. For more information see here.
 

Beta葡萄糖醛酸酶[大肠杆菌] β-Glucuronidase (E. coli) 货号:ME-BGLAEC 品牌:Megazyme


Beta葡萄糖醛酸酶[大肠杆菌]

英文名:β-Glucuronidase (E. coli)

货号:ME-BGLAEC

规格:500,000 Units

品牌: Megazyme

优惠价:2200.00 ( 原价3600.00 )

商品描述

High purity recombinant beta-Glucuronidase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.31
CAZy Family: GH2

Recombinant. From Escherichia coli. Supplied in solution (Tris.HCl / NaCl / EDTA).
Hydrolysis of non-reducing terminal β-D-glucuronic acid residues from glycoproteins and oligosaccharides of glycoconjugates.

Specific activity: ~ 35,000 U/mg (37oC, pH 6.8 on phenolphthalein-β-D-glucuronide); 
                           ~ 100 U/mg (37oC, pH 7.5 on pNP-β-D-glucuronide).

Store at 4oC. 
Do Not Freeze/Thaw 

Custom quantities and formulations available up on request.