- 详细信息
- 询价记录
- 相关实验
-
货号: 039-01732 供应商: 上海金畔生物科技有限公司 数量: 大量 英文名: Cerium(III) Acetate Monohydrate 规格: 25g 产品详情请咨询:上海金畔生物科技有限公司,021-50837765温馨提示:不可用于临床治疗。
货号: | 039-01732 |
供应商: | 上海金畔生物科技有限公司 |
数量: | 大量 |
英文名: | Cerium(III) Acetate Monohydrate |
规格: | 25g |
产品编号 | 产品名称 | 产品规格 | 产品等级 | 产品价格 |
AG-40B-0180B-C010 | Tim-4 (mouse):Fc (human) (rec.) (Biotin) Tim-4 (小鼠):Fc (人) (重组) (生物素标记) |
10 µg | – | – |
AG-40B-0180B-3010 | Tim-4 (mouse):Fc (human) (rec.) (Biotin) Tim-4 (小鼠):Fc (人) (重组) (生物素标记) |
3 x 10 µg | – | – |
Tim-4 (mouse):Fc (human) (rec.) (Biotin)
Tim-4(小鼠):Fc(人)(重组)(生物素标记)
◆产品详情
别名:TIM4,TIMD4
T Cell Immunoglobulin and Mucin Domain-containing Protein 4;
T细胞免疫球蛋白和粘蛋白结构域蛋白4
产品类型:蛋白质
特性
来源/宿主: CHO细胞
序列:小鼠TIM-4(aa 22-279)的胞外域融合到人IgG1的Fc区的N端
交叉反应性:人,小鼠
应用
功能:
-抑制受刺激的T细胞增殖
-用于分离细胞外囊泡
标记:生物素标记
特异性:TIM-4可以用于分离表面含有磷脂酰丝氨酸(PS)的不同物种(人,小鼠,大鼠等)的细胞外囊泡。
生物活性:测量其对经抗CD3诱导刺激人T细胞增殖能力的抑制作用。Tim-4 (mouse):Fc (human)(rec.)
(Biotin)结合磁珠可用于细胞外囊泡的分离。在钙离子依存的情况下,120ng蛋白足够分离
1010的细胞外囊泡颗粒。
分子量:~95kDa (SDS-PAGE)
纯度:≥95% (SDS-PAGE)
内毒素:<0.05EU/μg 蛋白 (LAL test; Lonza)
浓度:复溶后0.1mg/mL
复溶:100 µL灭菌水复溶
规格:溶于PBS中,经0.2μm过滤后冻干
其他产品数据:NCBI reference NP_848874.3: Tim-4 (mouse)
运输和保存
运输:冰块
短期储存:+4℃
长期储存:-20°C
保存建议:避免反复冻融。
使用/稳定性:收到产品后可在-20°C稳定保存6-12个月。
产品分装后可在-20°C稳定保存3个月。
◆产品描述
细胞外囊泡(EVs)由多种细胞释放到细胞微环境中,可以自发的运输或携带多种生物活性分子,如非编码RNA、miRNA、基因组DNA、脂质、生长因子和信号分子。EVs可分为外泌体(30~100nm)、微囊泡(100-1000 nm)和凋亡小体(>1000 nm)。EVs不仅在正常生理过程的调节中起着重要作用,而且在疾病发病机制中起着重要作用,它的内含物能够反映分泌细胞的生理状态。目前正在研究和开发利用EVs进行治疗和诊断的方法。因此,开发理想的EVs分离和定量方法已经成为了一个活跃的研究领域。细胞外囊泡在其外部脂质双分子层上表达磷脂酰丝氨酸(PS)。
Tim-4 (T cell immunoglobulin and mucin domain-containing protein 4)是一种单链I型膜蛋白,属于免疫球蛋白超家族和TIM家族。Tim-4含有一个Ig样V型(免疫球蛋白样)结构域。它在树突细胞和巨噬细胞上表达。Tim-4在T辅助细胞2(Th2)的增殖中起重要作用。Tim-4以钙离子依赖的形式与凋亡细胞表面的磷脂酰丝氨酸(PS)结合,并且介导凋亡细胞的吞噬。
EV膜富含磷脂酰丝氨酸(PS),Tim-4可以结合EVs表面上的PS。华山力成教授团队开发了一种新型的亲和方法,通过将生物素亲和的Tim-4结合到链霉素磁珠上,在钙离子存在的情况下,进行EVs的分离。该方法可以用于替代目前最常用的超速离心法来进行EVs的纯化。这种全新的Tim-4亲和法与其他方法相比(如超离法、PEG沉淀法、抗体免疫沉淀法),可以获得更好的产率、纯度,以及分离多种群EVs。
参考文献
[1] A novel affinity-based method for the isolation of highly purified extracellular vesicles:
W. Nakai, et al.; Sci. Rep. 6, 33935 (2016)
[2] High purity isolation and sensitive quantification of extracellular vesicles using affinity
to Tim-4: T. Yoshida, et al.; Curr. Prot. Cell Biol. 77, 3.45.1-3.45.18 (2017)
jQuery(“.nltabbox”).slide({effect:”fade”});
品牌 | 其他品牌 | 货号 | ADI-900-057 |
---|---|---|---|
规格 | 96wells | 供货周期 | 一个月 |
主要用途 | 残留蛋白A检测 |
残留A蛋白检测试剂盒(Protein A)
金黄色葡萄球菌细胞壁中的A蛋白(Staphylococal Protein A,SPA)又称Protein A,42kDa,能特异性地结合IgG抗体,通常在大规模抗体制备中用于纯化IgG。如果制备的抗体中存在Protein A,会严重降低抗体的质量。
残留A蛋白检测试剂盒(Protein A)
ENZO Life Sciences 强势推出的残留A蛋白检测试剂盒(Protein A)采用夹心法定量检测单抗制备过程中的Protein A残留,超灵敏,具有极好的批间重复性。
金黄色葡萄球菌细胞壁中的A蛋白(Staphylococal Protein A,SPA)又称Protein A,42kDa,能特异性地结合IgG抗体,通常在大规模抗体制备中用于纯化IgG。如果制备的抗体中存在Protein A,会严重降低抗体的质量。
特点:
产品编号 | 产品名称 | 规格 |
ADI-900-057 |
Protein A ELISA Kit
|
96孔 |
与其他品牌的同类产品对比,金畔.中国代理ENZO Life Sciences的Protein A ELISA KIT产品,灵敏度明显更优,价格却实惠30%甚至50%。
详情请咨询:金畔.中国020-8732 6381 /8735 3867,也可回复本邮件进一步沟通,期待您的回复。
温馨提示:不可用于临床治疗。
L-鼠李糖检测试剂盒
英文名:L-Rhamnose Assay Kit
货号:K-RHAMNOSE
规格:50 / 100 assays (manual) / 550 assays
This product (K-RHAMNOSE) supersedes the original L-Rhamnose Assay Kit (K-RHAM) which has been discontinued. K-RHAMNOSE provides a more rapid reaction (~ 5 min.) and much improved reagent stability compared to the previous kit.
The L-Rhamnose test kit is a simple, rapid, reliable and accurate method for the measurement of L-rhamnose in plant extracts, culture media/supernatants and other materials.
Suitable for manual, auto-analyser and microplate formats.
UV-method for the determination of L-Rhamnose in hydrolysates
of plant material, polysaccharides, culture media / supernatants
and other materials. Suitable for use with manual, microplate
and auto-analyser formats
Principle:
(L-rhamnose dehydrogenase)
(1) L-Rhamnose + NAD+ → L-rhamno-1,4-lactone + NADH + H+
Kit size: 50 / 100 assays (manual) / 550 (microplate)
/ 550 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 5 min at 25°C or ~ 4 min at 37°C
Detection limit: ~ 1.2 mg/L
Application examples:
Hydrolysates of plant material and polysaccharides, culture media /
supernatants and other materials
Method recognition: Novel method
Advantages
Q1. Should the pH of the sample be adjusted even for samples in acidic media?
The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.
Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.
If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:
For samples with low concentrations of analyte the sample volume used in the kit assay can be increased to increase sensitivity. When doing this the water volume is adjusted to retain the same final assay volume. This is critical for the manual assay format because the assay volume and sample volume are used in the calculation of results.
The volume/weight of sample and total volume of the extract can be modified to suit the sample. This will ultimately be dictated by the amount of analyte of interest in the sample and may require empirical determination. For low levels of analyte the sample:extract volume ratio can be increased (i.e. increase the sample and/or decrease the total extraction volume).
Alternatively, for samples with low concentrations of analyte, a larger sample volume can be added to the kit assay. When altering the sample volume adjust the distilled water volume added to the assay accordingly so that the total assay volume is not altered.
The kit assay may work for biological fluids assuming that inositol is present above the limit of detection for the kit after any sample preparation (if required). Centrifugation of the samples and use of the supernatant directly in the kit assay (with appropriate dilution in distilled water) may be sufficient. However, if required a more stringent sample preparation method may be required and examples are provided at the following link:http://www.megazyme.com/docs/analytical-applications-downloads/biological_samples_111109.pdf?sfvrsn=2
The test kit has not been tested using biological fluids as samples because it is not marketed or registered as a medical device. This will therefore require your own validation.
The majority of the Megazyme test kits are developed to work in cuvettes using the manual assay format, however the assay can be converted for use in a 96-well microplate format. To do this the assay volumes for the manual cuvette format are reduced by 10-fold. The calculation of results for the manual assay format uses a 1 cm path-length, however the path-length in the microplate is not 1 cm and therefore the MegaCalc spreadsheet or the calculation provided in the kit booklet for the manual format cannot be used for the micropalate format unless the microplate reader being used can.
There a 3 main methods for calculation of results using the microplate format:
Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.
If there are any concerns with any kit components, the first thing to do is to test the standard sample (control sample) that is supplied with the kit and ensure that the expected value (within the accepted variation) is obtained before testing any precious samples. This must be done using the procedure provided in the kit booklet without any modifications to the procedure. If there are still doubts about the results using the standard sample in the kit then send example results in the MegaCalc spread sheet to your product supplier (Megazyme or your local Megazyme distributor).
The kit assay will only measure the non-covalently linked monosaccharide.
Oligosaccharides or polysaccharides can be measured after hydrolysis to the monosaccharide. Generally acid hydrolysis can be achieved by boiling the oligo/polysaccharide in 1.3 M HCl for 1 h. It is recommended that scientific literature is consulted for information on hydrolysis conditions for the particular oligo/polysaccharide that is being measured.
No. The 0.1 change of absorbance is only a recommendation. The lowest acceptable change in absorbance can is dictated by the analyst and equipment (i.e. pipettes and spectrophotometer) and therefore can be can be determined by the user. With accurate pipetting, absorbance changes as low as 0.02 can be used accurately.
If a change in absorbance above 0.1 is required but cannot be achieved due to low concentrations of analyte in a sample, this can be overcome by using a larger sample volume in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results.
Yes. Samples with the lower concentrations of analyte will generate a lower absorbance change. For samples with low concentrations of analyte, a larger sample volume can be used in the assay to increase the absorbance change and thereby increase sensitivity of the assay. When doing this the increased volume of the sample should be subtracted from the distilled water volume that is added to the assay so that the total assay volume is unaltered. The increase sample volume should also be accounted for when calculating final results.
The test kit is extremely accurate – at Megazyme the quality control criteria for accuracy and repeatability is to be within 2% of the expected value using pure analytes.
However, the level of accuracy is obviously analyst and sample dependent.
Yes, instead of adding 2 μL of enzyme suspension an alternative is to dilute the enzyme and add a larger volume to the microplate assay.
Dilute the assay buffer 10-fold with distilled water and use this as the diluent to dilute an aliquot of the enzyme suspension also by 10-fold. Instead of 2 μL, use 20 μL of the diluted enzyme in the microplate assay.
PALL 颇尔过滤器PALL GHP万能针头滤器 Acrodisc针头过滤器4563 过滤介质:亲水型聚丙烯外壳:聚丙烯。玻纤预滤层:硼硅玻璃
标准死体积:13mm:<14ul;
25mm:<100ul;25mm GF:<125ul
有效过滤面积:13/25mm;0.8/2.8cm2
PALL 颇尔过滤器PALLPALL GHP膜Acrodisc针头过滤器和圆盘过滤膜片
特点:
PALL独有的GHP材质,可用于水和腐蚀性有机溶剂的溶液,过滤酸,碱,醇等等,几乎所有的溶液,所有有万能过滤膜之称。
它使用AUtoPackTM包装方便使用,具有Caliper自动化认证,与多种自动化工作站兼容。
它的低蛋白结合膜保证低蛋白质样品的回收率很高。
精确分析,HPLC认证,紫外线吸收萃取物水平低。
使用带玻璃纤维预滤器易于直接过滤颗粒样品。
带有Minispike出口的13mmAcrodisc针头过滤器提供低残留量,易于过滤到自动取样小瓶中。
PALL 颇尔过滤器PALL应用:
强烈推荐用于过滤HPLC样品和流动相;
过滤极度粘性样品时,AcrodiscPSF GxF 针头过滤器的过滤能力是标准预过滤器的2-4倍。
产品信息:
货号 | 描述 | 包装 | ||
Acrodisc® Syringe Filters with GHP Membrane, 13 mm | ||||
---|---|---|---|---|
4554 | 0.2 µm, minispike outlet | 100/pkg 300/cs | ||
4567 | 0.2 µm, minispike outlet | 1000/pkg | ||
4556 | 0.45 µm, minispike outlet | 100/pkg 300/cs | ||
4563 | 0.45 µm, minispike outlet | 1000/pkg | ||
Acrodisc Syringe Filters With GHP Membrane, 25 mm | ||||
4564 | 0.2 μm | 50/pkg, 200/cs | ||
4566 | 0.2 μm | 1000/pkg | ||
4560 | 0.45 μm | 50/pkg, 200/cs | ||
4562 | 0.45 μm | 1000/pkg | ||
Acrodisc GF Syringe Filters With GHP Membrane, 25 mm | ||||
4559 | GF/0.45 μm | 50/pkg, 200/cs | ||
4558 | GF/0.45 μm | 1000/pkg | ||
Acrodisc PSF Syringe Filters with GHP Membrane, 25 mm | ||||
AP-4364 | 0.2 µm, AutoPack™ tubes | 25/pkg 200/cs | ||
AP-4564 | 0.2 µm | 50/pkg 200/cs | ||
AP-4566 | 0.2 µm | 1000/pkg | ||
AP-4357 | 0.45 µm, AutoPack tubes | 25/pkg 200/cs | ||
AP-4560 | 0.45 µm | 50/pkg 200/cs | ||
AP-4562 | 0.45 µm | 1000/pkg | ||
Acrodisc PSF GxF Syringe Filters with GHP Membrane, 25 mm | ||||
AP-4305 | GxF/0.2 µm, AutoPack tubes | 25/pkg 200/cs | ||
AP-4307 | GxF/0.2 µm | 50/pkg 200/cs | ||
AP-4306 | GxF/0.2 µm | 1000/pkg | ||
AP-4557 | GxF/0.45 µm, AutoPack tubes | 25/pkg 200/cs | ||
AP-4559 | GxF/0.45 µm | 50/pkg 200/cs | ||
AP-4558 | GxF/0.45 µm | 1000/pkg |